منابع مشابه
Intercellular delivery of a herpes simplex virus VP22 fusion protein from cells infected with lentiviral vectors.
Effective gene therapy depends on the efficient transfer of therapeutic genes and their protein products to target cells. Lentiviral vectors appear promising for virus-mediated gene delivery and long-term expression in nondividing cells. The herpes simplex virus type 1 tegument protein VP22 has recently been shown to mediate intercellular transport of proteins, raising the possibility that it m...
متن کاملConformational lability of herpesvirus protein VP22.
The herpesvirus protein VP22 traffics between cells, being exported from expressing cells in a non-Golgi-dependent manner and localizing in the nuclei of surrounding cells. This transport is retained in certain VP22 fusion proteins, making VP22 a candidate for use in macromolecular drug delivery. In an effort to understand the physical basis for this activity, we have initiated structural studi...
متن کاملParticle formation by a conserved domain of the herpes simplex virus protein VP22 facilitating protein and nucleic acid delivery.
VP22, a structural protein of herpes simplex virus, exhibits unusual trafficking properties which we proposed might be exploited in gene and protein delivery applications. To pursue the use of the protein itself for cargo delivery into cells, we developed an expression system for the C-terminal half of VP22, residues 159-301 (VP22.C1), and purified the protein in high yields. Addition of short ...
متن کاملPeptide and Protein Delivery at a Glance
Peptide and protein drugs have found an important position in therapeutics. Recent advances in pharmaceutical biotechnology have led to an increase in the number of protein products in the market. As these therapeutic proteins and peptides are made available, it will be essential to formulate these drugs into safe and effective delivery systems. The twenty different naturally occurring amin...
متن کاملIntercellular Trafficking of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
The herpes simplex virus type 1 (HSV-1) tegument protein, VP22 has been reported to have the property of intercellular transport. The previous studies have shown that following expression of a fusion protein containing VP22 it spreads to every cell in a monolayer and concentrates in the nucleus. In spite of these reports, some studies have shown that VP22 trafficking and its nucleus accumulatio...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2002
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt0102-20